がん免疫療法 - 自家がんワクチン

　この度、2011年4月2-6日開催のアメリカ癌学会（AACR 102nd Annual Meeting 2011 in Orlando、フロリダ）から、JA尾道総合病院・倉西文仁先生他11名の共著の演題申し込みが受理されたとの知らせがありました。弊社も共同研究させていただいております。

　今回は、術後乳がん症例32例に自家がんワクチンを投与した場合、27例（84％）でDTH反応が陽転し、陽転群では末梢血T細胞中で免疫反応の重要な指標が有意に上昇するという結果を報告します。

　発表のセッション、時間、場所、抄録は以下のとおりです。
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Session ID: Immunology 8
Session Date and Time: Wednesday Apr 6, 2011 8:00 AM - 12:00 PM
Location: Exhibit Hall A4-C, Poster Section 32
Permanent Abstract Number: 5522

Title:
Increase of Th1/Treg ratio in peripheral blood of breast cancer
patients with autologous formalin-fixed tumor vaccine

Authors:
Fumito Kuranishi1, Yoshinori Kuroda1, Toshiyuki Noriyuki1, Masahiro Nakahara1, Minoru Yamaki1, Daisuke Sumitani1, Kiyomi Shimoda1, Asuka Tanaka1, Michinori Hamaoka1,Kazuhiro Taguchi1, Gen Takahashi 1 and Tadao Ohno2

Affiliation:
1 Department of Surgery, Onomichi General Hospital, Onomichi, Hiroshima 722-8508 and 2 Cell-Medicine, Inc., 2-1 Sengen, Tsukuba Science City, Ibaraki 305-0074, Japan

Introduction: Autologous formalin-fixed tumor vaccine (AFTV) containing formalin-fixed and/or paraffin-embedded tumor fragments is able to induce tumor specific CTL and suppresses recurrence of hepatocellular carcinoma after resection (Clin Cancer Res 2004; 10: 1574-9). We have used delayed-type hypersensitivity (DTH) test as a parameter of cellular immune response. We also found that slight but consistent population change has occurred in peripheral blood T lymphocytes after AFTV treatment.

Method: Thirty two breast cancers were operated in our hospital. AFTV was injected intradermally. DTH response to the tumor fragments without any adjuvant was determined if erythema was 10mm or more.

Results: After operation, standard chemotherapy, and enough time have passed to recover immunological function, we examined change of peripheral blood T-cell population in 32 cases with breast cancer. Among them, 27 cases turned DTH response from negative to positive after AFTV injection. In these patients, WBC counts increased 1.11 times; lymphocytes, 1.09 times; Th1 in CD4+ T cells, 1.07 times; regulatory T cells (Treg) in CD4+ T cells, 0.95 times, and Th1/Treg ratio, 1.17 times (statistically significant, 95%CI was 1.060-1.284).

Conclusion: AFTV probably strengthen the cellular immune response against cancer by improving Th1/Treg ratio in peripheral blood.
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